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Recombinant Mouse Cytochrome P450 7A1 (Cyp7a1)

Cat. No.AP4C739

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Mouse Cytochrome P450 7A1 (Cyp7a1) is a recombinant protein expressed from E.coli. Its predicted molecular weight is 31.5 kDa. The purity is> 95%.

Specifications

Type Recombinant Protein
Species Mouse
Expression System E.coli
Purity > 95%
Endotoxin <1.0 EU per 1µg (determined by the LAL method)
Predicted Molecular Weight 31.5 kDa
Physical State Lyophilized
Formulation PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.

Target Information

Mouse Cytochrome P450 7A1 (Cyp7a1) is a key enzyme in the bile acid synthesis pathway, responsible for the conversion of cholesterol to 7α-hydroxycholesterol. This enzyme is a member of the cytochrome P450 family and plays a crucial role in regulating cholesterol levels and maintaining bile acid homeostasis. Cyp7a1 is highly expressed in the liver and is regulated by various factors, including bile acids themselves through the farnesoid X receptor (FXR) and the liver X receptor (LXR) . Hypoxic conditions can downregulate Cyp7a1 expression through the hypoxia-inducible factor-1α (HIF-1α) pathway, which limits the availability of its substrate, oxygen, and decreases transcription .

Protein Mouse Cytochrome P450 7A1 (Cyp7a1)
Protein Synonym 24-hydroxycholesterol 7-alpha-hydroxylase; CYPVII; Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase
Gene ID 13122
EC No. Q64505

Shipping and Storage

This product is shipped with ice packs. Lyophilized protein can be stored at -80°C for 1 year. After reconstitution, the protein solution can be stored at 2-8°C for 2-7 days.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in PBS or others. Aliquot the reconstituted solution to minimize freeze-thaw cycles.
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