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Recombinant Canine Tumor Necrosis Factor Alpha (TNF), N-His
Product Overview
BioVenic's Recombinant Canine Tumor Necrosis Factor Alpha (TNF), N-His is a recombinant protein expressed from E.coli expression system. Its predicted molecular weight is 20.2 kDa. The purity is>97% (SDS-PAGE). The endotoxin level is <1 EU/μg (LAL). It can be used as a positive control or as an immunogen.
Specifications
Target Information
Tumor necrosis factor, also known as TNF-α, is encoded by the TNF gene in dogs. It is a marker of inflammation which was significantly higher in dogs with congestive heart failure (CHF) compared to dogs without CHF.
Shipping and Storage
This product is shipped with dry ice. Avoid repeated freezing and thawing. Store for 4 weeks at 2-8°C, for 12 months at -80°C.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Aliquote the reconstituted solution to minimise freeze-thaw cycles.
References
- Nemec Svete, A. et al. Inflammation and its association with oxidative stress in dogs with heart failure. BMC Veterinary Research. 2021, 17: 176.