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Recombinant Bovine Pancreatic Trypsin Inhibitor, N-His

Cat. No.AP9C449

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Bovine Pancreatic Trypsin Inhibitor, N-His is a recombinant protein expressed from E.coli. Its predicted molecular weight is 10.2 kDa. The purity is>95% (SDS-PAGE).

Specifications

Type Recombinant Protein
Species Bovine
Expression System E.coli
Purity >95% (SDS-PAGE)
Endotoxin < 1 EU/µg
Predicted Molecular Weight 10.2 kDa
Molecular Weight 14 kDa
Physical State Lyophilized
Formulation The buffer before lyophilization is 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Target Information

Bovine pancreatic trypsin inhibitor (PTI), also known as aprotinin or Bovine basic pancreatic trypsin inhibitor (BPTI), is a protein that belongs to the Kunitz family of serine protease inhibitors. It is a small, compactly folded, globular protein of 58 amino-acid residues with a mass of 6512 Da, stabilized by three disulfide bonds. PTI is found in a variety of organs and tissues in mammals, including Bovine, and also in other vertebrates and invertebrates. It has a relatively broad specificity, inhibiting several kinds of digestive enzymes such as trypsin, chymotrypsin, plasmin, and kallikrein.

Protein Bovine Pancreatic Trypsin Inhibitor
Protein Synonym BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor
Gene ID 404172
UniProt ID P00974

Shipping and Storage

This product is shipped with dry ice. It is recommended to aliquot as needed and store at -80°C upon receipt. Reconstituted protein solution can be stored at 4°C for 1 week, at < -80°C for 12 months. Avoid repeated freezing and thawing.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimise freeze-thaw cycles.
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