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Recombinant Bovine Collagenase 3 (MMP13), N-His

Cat. No.AP9C360

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Bovine Collagenase 3 (MMP13), N-His is a recombinant protein expressed from E.coli. Its predicted molecular weight is 28.2 kDa. The purity is>90% (SDS-PAGE).

Specifications

Type Recombinant Protein
Species Bovine
Expression System E.coli
Purity >90% (SDS-PAGE)
Endotoxin < 1 EU/µg
Predicted Molecular Weight 28.2 kDa
Molecular Weight 32 kDa
Physical State Lyophilized
Formulation The buffer before lyophilization is 20 mM Tris, 150 mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Target Information

Bovine matrix metalloproteinase 13 (MMP13), also known as collagenase-3, is a member of the matrix metalloproteinase family of enzymes. This protein is essential in cattle for the degradation of extracellular matrix components, particularly collagen types I, II, and III, which are crucial for bone and cartilage health. MMP13 plays a significant role in physiological processes such as tissue remodeling, wound healing, and the development of the skeletal system. It is highly expressed during embryogenesis and in adult bone during endochondral ossification. In the context of disease, MMP13 is implicated in the pathogenesis of conditions like osteoarthritis and is a key regulator in the activation of osteoclasts, which are important for bone resorption. The balanced expression and activity of MMP13 are vital for maintaining tissue homeostasis, and its dysregulation can lead to various pathological conditions in cattle.

Protein Bovine Collagenase 3 (MMP13)
Protein Synonym CLG3; Collagenase 3
Gene ID 281914
UniProt ID O77656

Shipping and Storage

This product is shipped with dry ice. It is recommended to aliquot as needed and store at -80°C upon receipt. Reconstituted protein solution can be stored at 4°C for 1 week, at < -80°C for 12 months. Avoid repeated freezing and thawing.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimise freeze-thaw cycles.
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