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Rat 25-Hydroxycholesterol (25-HC) ELISA Kit-Competitive

Cat. No.EK4F28

Product TypeAnimal Immunoassay Kits

Size 96T

Product Overview

BioVenic Rat 25-Hydroxycholesterol (25-HC) ELISA Kit-Competitive is designed for the quantitative determination of Rat 25-HC in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Competitive ELISA method. For research use only.

Specifications

Assay Type ELISA-Competitive
Specificity The assay kit is specific for Rat 25-HC.
Target Species Rat
Species Reactivity Rat
Detection Range 1-50 μg/L
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

CH25H is an intronless gene encoding a membrane-bound enzyme that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25-HC). It plays a pivotal role in lipid metabolism and innate immunity. By producing 25-HC, it regulates sterile inflammation, inhibits viral entry (including enveloped viruses), and modulates B-cell antibody production. It acts as a critical link between metabolic pathways and immune signaling, making it a key target in inflammatory diseases and antiviral research.

Target/Biomarker Rat 25-HC
Target Synonym 25-hydroxycholesterol; 25-HC; 25-OHC

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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