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Rabbit Anti-Mouse Autophagy Related Protein 7 (ATG7) Polyclonal Antibody
Product Overview
BioVenic rabbit polyclonal antibody is specific for mouse autophagy related protein 7. It is purified by protein A. It can be applied to WB, IHC, ICC and IP assays of mouse autophagy related protein 7.
Specifications
Target Information
Mouse autophagy-related protein 7 (Atg7) is a 78 kDa E1-like enzyme that orchestrates the lipidation cascade essential for autophagosome expansion. Its N-terminal adenylation domain activates Atg12 and LC3 in succession, while the C-terminal ubiquitin-fold recruits the Atg10 and Atg3 E2 enzymes to catalyze two parallel conjugation pathways. A flexible linker between residues 400-450 swings the catalytic cysteine between donor and acceptor sites, enabling sequential transfer without substrate release. Polyclonal antibodies raised against the unique linker region detect both cytosolic and membrane-bound Atg7, distinguishing full-length from calpain-cleaved fragments in tissue lysates and immunofluorescence assays.
Shipping and Storage
This product is shipped with wet ice packs. Store at -20°C on receipt (up to 12 months). Avoid repeated freezing and thawing as this may denature the antibody.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.