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Porcine Nicotinamide Phosphoribosyltransferase (NAMPT) ELISA Kit-Sandwich

Cat. No.EK12F437

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Porcine Nicotinamide Phosphoribosyltransferase (NAMPT) ELISA Kit-Sandwich is designed for the quantitative determination of Porcine Nicotinamide Phosphoribosyltransferase (NAMPT) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Porcine NAMPT.
Target Species Swine
Species Reactivity Swine
Detection Range 3.12-200 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%
Assay Time Around 270 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

Nicotinamide phosphoribosyltransferase (NAMPT), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin (for its extracellular form), is an enzyme encoded by the NAMPT gene in pigs. NAMPT catalyzes the transfer of a phosphoribosyl group from 5-phosphoribosyl-1-pyrophosphate (PRPP) to nicotinamide, forming nicotinamide mononucleotide (NMN). NMN is a key intermediate in the NAD pathway, which is essential for various cellular processes. It is the rate-limiting enzyme in the biosynthesis of nicotinamide adenine dinucleotide.

Target/Biomarker Porcine NAMPT
Target Synonym Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1 homolog; PBEF; Visfatin; NAMPT; PBEF1; 2.4.2.12
Gene ID 595123
UniProt ID Q52I78

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Szymanska, K. et al. The Effect of Visfatin on the Functioning of the Porcine Pituitary Gland: An In Vitro Study. Cells. 2023, 12: 2835.
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