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Porcine 25-Hydroxycholesterol (25-HC) ELISA Kit-Competitive

Cat. No.EK4F30

Product TypeAnimal Immunoassay Kits

Size 96T

Product Overview

BioVenic Porcine 25-Hydroxycholesterol (25-HC) ELISA Kit-Competitive is designed for the quantitative determination of Swine 25-HC in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Competitive ELISA method. For research use only.

Specifications

Assay Type ELISA-Competitive
Specificity The assay kit is specific for Swine 25-HC.
Target Species Swine
Species Reactivity Swine
Detection Range 1-50 μg/L
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

In swine, CH25H is a crucial interferon-stimulated gene (ISG) that encodes the enzyme converting cholesterol to 25-hydroxycholesterol (25-HC). It serves as a potent restrictor of major porcine viral pathogens, including PRRSV and ASFV, by altering membrane fluidity and inhibiting viral fusion. Beyond its antiviral properties, porcine CH25H regulates lipid metabolism and inflammatory signaling in alveolar macrophages, making it a primary focus in veterinary immunology and the development of resistant livestock breeds.

Target/Biomarker Swine 25-HC
Target Synonym 25-hydroxycholesterol; 25-HC; 25-OHC

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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