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Ovine Secreted Phosphoprotein 1 (SPP1) ELISA Kit-Sandwich

Cat. No.EK8F151

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Ovine Secreted Phosphoprotein 1 (SPP1) ELISA Kit-Sandwich is designed for the quantitative determination of Ovine SPP1 in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Ovine SPP1.
Target Species Ovine
Species Reactivity Ovine
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

Secreted phosphoprotein 1 is encoded by the SPP1 gene in sheep. It is also known as osteopontin (OPN). OPN is an acidic glycoprotein that fragments when subjected to freezing and thawing or treated with proteases such as thrombin. It functions as a secreted extracellular matrix (ECM) protein and interacts with various cell surface integrins to promote cell-cell and cell-ECM adhesion and communication. It is widely acknowledged that OPN engages with integrin receptors expressed at the apical surface of the uterine luminal epithelium (LE) and the conceptus trophectoderm, facilitating the attachment of the conceptus to the uterus for implantation.

Target/Biomarker Ovine SPP1
Target Synonym secreted phosphoprotein 1; SPP1; OPN; osteopontin; SPP-1; bone sialoprotein 1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
Gene ID 443058
UniProt ID Q9XSY9

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Johnson, Greg A., Robert C. Burghardt, and Fuller W. Bazer. "Osteopontin: a leading candidate adhesion molecule for implantation in pigs and sheep." Journal of animal science and biotechnology 5 (2014): 1-14.
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