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Mouse Xanthine Dehydrogenase (XDH) ELISA Kit-Sandwich

Cat. No.EK6F248

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Xanthine Dehydrogenase (XDH) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse XDH in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Xanthine Dehydrogenase (XDH).
Target Species Mouse
Species Reactivity Mouse
Detection Range 31.25-2000 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Xanthine dehydrogenase/oxidase (XDH) is a crucial enzyme in purine degradation, catalyzing the oxidation of hypoxanthine to xanthine and xanthine to uric acid. It also contributes to the generation of reactive oxygen species. In mice, XDH has diverse biological functions, including roles in purine metabolism, antioxidant activity, inflammatory responses, and tissue repair. Mutations in the Xdh gene are linked to early renal failure (RENF) in mice, resulting in the loss of XDH activity in the liver and kidneys. This leads to urate deposition in the renal tubules, triggering renal fibrosis and interstitial inflammation. These findings underscore the importance of XDH in maintaining renal health and its broader role in metabolic and inflammatory processes.

Target/Biomarker Mouse XDH
Target Synonym XO; XOR; XOD; Xanthine Oxidase; NAD+-Xanthine Dehydrogenase; Xanthine-NAD+ Oxidoreductase; Xanthine/NAD+ Oxidoreductase; Xanthine Oxidoreductase
Gene ID 22436
UniProt ID Q00519

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Piret, Sian E et al. "A mouse model of early-onset renal failure due to a xanthine dehydrogenase nonsense mutation." PloS one vol. 7,9 (2012): e45217.
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