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Mouse WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA Kit-Sandwich

Cat. No.EK5F246

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse WISP1 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse WNT1 Inducible Signaling Pathway Protein 1 (WISP1).
Target Species Mouse
Species Reactivity Mouse
Detection Range 6.25-400 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

CCN family member 4 (CCN4) acts as a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. It adheres to skin and melanoma fibroblasts, with in vitro binding to skin fibroblasts occurring through the proteoglycans decorin and biglycan. In mice, CCN4 has diverse roles in tissue remodeling, tumor progression, vascular biology, bone metabolism, fibrosis, and arthritis. In a prostate cancer model, CCN4 expression is linked to tumor growth and bone metastasis. The use of anti-CCN4 antibodies can inhibit bone metastasis of PC-3-Luc cells, highlighting CCN4's significant role in the early development and bone metastasis of prostate cancer.

Target/Biomarker Mouse WISP1
Target Synonym CCN4; WISP1c; WISP1i; WISP1tc; CCN family member 4; Wnt-1-induced secreted protein
Gene ID 22402
UniProt ID O54775

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Ono, Mitsuaki et al. "WISP1/CCN4: a potential target for inhibiting prostate cancer growth and spread to bone." PloS one vol. 8,8 e71709. 14 Aug. 2013.
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