Mouse Ubiquitin Conjugating Enzyme E2I (UBE2I) ELISA Kit-Sandwich

The SUMO-conjugating enzyme UBC9 facilitates the acceptance of ubiquitin-like proteins SUMO1, SUMO2, and SUMO3 from the UBLE1A-UBLE1B E1 complex and aids in their covalent attachment to other proteins, with the assistance of E3 ligases such as RANBP2, CBX4, and ZNF451. UBC9 is capable of catalyzing the formation of poly-SUMO chains and is crucial for maintaining nuclear architecture, ensuring proper chromosome segregation, and supporting embryonic viability. It plays a vital role in the sumoylation of proteins like FOXL2 and KAT5. In mouse oocytes, UBE2I is primarily involved in transcriptional regulation and chromatin remodeling. Research indicates that UBE2I is localized in nuclear speckles and co-localizes with the SFRS2 protein, which is essential for RNA processing. Furthermore, UBE2I promotes normal oocyte development by enhancing transcriptional activity, a function that is independent of its catalytic activity.