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Mouse Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) ELISA Kit-Sandwich

Cat. No.EK6F678

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse TNFaIP6 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6).
Target Species Mouse
Species Reactivity Mouse
Detection Range 78.25-5000 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

The tumor necrosis factor-inducible gene 6 protein plays a crucial role in regulating the organization of the extracellular matrix during the process of tissue remodeling. It facilitates the transfer of a heavy chain (HC) from the inter-alpha-inhibitor (I-alpha-I) complex to hyaluronan. This involves cleaving the ester bond between the C-terminus of the HC and the GalNAc residue of the chondroitin sulfate chain within the I-alpha-I complex, followed by the transesterification of the HC to hyaluronan. This mechanism enhances the antiprotease function of the I-alpha-I complex by releasing free bikunin. Mice that lack TNFAIP6 show intensified inflammatory responses in antigen-induced arthritis, characterized by more pronounced synovial infiltration, destruction of articular cartilage, and bone erosion.

Target/Biomarker Mouse TNFaIP6
Target Synonym TSG6; TNF-Stimulated Gene 6; Hyaluronate-binding protein; Tumor necrosis factor alpha-induced protein 6
Gene ID 21930
UniProt ID O08859

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Rychkov, Dmitry et al. "Cross-Tissue Transcriptomic Analysis Leveraging Machine Learning Approaches Identifies New Biomarkers for Rheumatoid Arthritis." Frontiers in immunology vol. 12 638066. 8 Jun. 2021.
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