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Mouse Tryptophan Hydroxylase 2 (TPH2) ELISA Kit-Sandwich

Cat. No.EK6F213

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Tryptophan Hydroxylase 2 (TPH2) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse TPH2 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Tryptophan Hydroxylase 2 (TPH2).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

In mice, tryptophan hydroxylase 2 (Tph2) serves as the rate-limiting enzyme for synthesizing 5-hydroxytryptamine (5-HT) in the central nervous system. Tph2 is primarily expressed in the raphe nuclei of the brainstem and is responsible for catalyzing the conversion of tryptophan to 5-hydroxytryptophan, a crucial step in 5-HT synthesis. Functional defects in Tph2 result in substantially decreased 5-HT levels in the brain, which are closely associated with various behavioral and mood disorders. For instance, Tph2 knockout mice display symptoms such as depressive-like behavior, anxiety-like behavior, and increased aggression. Additionally, mice with diminished Tph2 function also exhibit resistance to specific drug treatments, including antidepressants.

Target/Biomarker Mouse TPH2
Target Synonym NTPH; Neuronal tryptophan hydroxylase; Tryptophan 5-monooxygenase 2
Gene ID 216343
UniProt ID Q8CGV2

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Evsiukova, Valentina S et al. "Tryptophan Hydroxylase 2 Deficiency Modifies the Effects of Fluoxetine and Pargyline on the Behavior, 5-HT- and BDNF-Systems in the Brain of Zebrafish (Danio rerio)." International journal of molecular sciences vol. 22,23 12851. 27 Nov. 2021.
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