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Mouse Topoisomerase I (TOP1) ELISA Kit-Sandwich

Cat. No.EK6F438

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Topoisomerase I (TOP1) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse TOP1 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Topoisomerase I (TOP1).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Topoisomerase I alleviates the supercoiling and torsional tension of DNA that occurs during replication and transcription by temporarily cleaving and rejoining one strand of the DNA duplex. It achieves this by introducing a single-strand break via transesterification at a specific site in the DNA. In mice, Top1 binds to DNA to form a topoisomerase I complex (Top1cc), which facilitates the breakage of single-stranded DNA and subsequently relieves DNA superhelical tension. Furthermore, Top1 plays a significant role in the dynamic regulation of chromatin, particularly during neuronal development. Its absence can lead to the accumulation of DNA double-strand breaks (DSBs), which can trigger neurodegenerative diseases.

Target/Biomarker Mouse TOP1
Target Synonym TOPI; DNA Topoisomerase 1
Gene ID 21969
UniProt ID Q04750

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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