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Mouse Steroid 5 Alpha Reductase 1 (SRD5a1) ELISA Kit-Sandwich

Cat. No.EK6F680

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Steroid 5 Alpha Reductase 1 (SRD5a1) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse SRD5a1 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Steroid 5 Alpha Reductase 1 (SRD5a1).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

The enzyme 3-oxo-5-alpha-steroid 4-dehydrogenase 1 transforms testosterone into 5-alpha-dihydrotestosterone and converts progesterone or corticosterone into their respective 5-alpha-3-oxosteroids. It is crucial for sexual differentiation and androgen physiology. The Srd5a1 gene is highly expressed in the mouse liver, and its deletion leads to reduced cortisol clearance, resulting in a phenotype similar to "relative adrenal insufficiency." In this condition, while ACTH and cortisol levels remain normal, there is a diminished response to ACTH and stress stimuli, which could be linked to abnormal negative feedback regulation of the hypothalamus-pituitary axis.

Target/Biomarker Mouse SRD5a1
Target Synonym 3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 1
Gene ID 78925
UniProt ID Q68FF9

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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