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Mouse Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit-Sandwich

Cat. No.EK5F955

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse SFRP5 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Secreted Frizzled Related Protein 5 (SFRP5).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.25-16 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Soluble frizzled-related proteins (sFRPs) modulate Wnt signaling by directly interacting with Wnts, playing a crucial role in regulating cell growth and differentiation in specific cell types. SFRP5 may influence the polarity of photoreceptor cells and possibly other retinal cells. In mice, Sfrp5 exhibits significant metabolic regulatory, anti-inflammatory, and cardioprotective effects. It aids in improving obesity-related metabolic disorders and inflammatory responses by inhibiting the non-classical activation of the Wnt signaling pathway, and offers protection during cardiac ischemia/reperfusion injury. In mice on a high-fat diet, decreased expression of Sfrp5 leads to reduced insulin sensitivity, worsened fatty liver, and increased macrophage infiltration.

Target/Biomarker Mouse SFRP5
Target Synonym SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b
Gene ID 54612
UniProt ID Q9WU66

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Carstensen, Maren et al. "Effect of Sfrp5 on cytokine release and insulin action in primary human adipocytes and skeletal muscle cells." PloS one vol. 9,1 e85906. 21 Jan. 2014.
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