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Mouse Quinolinate Phosphoribosyltransferase (QPRT) ELISA Kit-Sandwich

Cat. No.EK6F576

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Quinolinate Phosphoribosyltransferase (QPRT) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse QPRT in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Quinolinate Phosphoribosyltransferase (QPRT).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.32-20 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Nicotinate-nucleotide pyrophosphorylase [carboxylating] plays a role in the catabolism of quinolinic acid (QA). In QPRT knockout (KO) mice, the absence of QPRT activity hinders effective QA degradation, leading to its accumulation in the body. This build-up can induce neurotoxicity, as QA is an excitotoxin for the NMDA receptor, potentially causing neuronal damage. Research has shown that QA levels significantly increase in the striatum of QPRT KO mice, accompanied by neuronal degeneration. These mice exhibit decreased motor and cognitive functions, such as reduced activity in the merry-go-round test, diminished spontaneous alternation behavior in the Y-maze test, and prolonged escape latency in the Barnes maze test.

Target/Biomarker Mouse QPRT
Target Synonym QPRTase; Nicotinate-Nucleotide Pyrophosphorylase(Carboxylating)
Gene ID 67375
UniProt ID Q91X91

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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