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Mouse Pyruvate Dehydrogenase Phosphatase (PDP) ELISA Kit-Sandwich

Cat. No.EK6F888

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Pyruvate Dehydrogenase Phosphatase (PDP) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse PDP in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Pyruvate Dehydrogenase Phosphatase (PDP).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Pyruvate Dehydrogenase Phosphatase is a mitochondrial enzyme responsible for the dephosphorylation and reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex (PDC). This reactivation encourages the conversion of pyruvate to acetyl-CoA. PDP1 serves as a phosphorylation regulator for PDC, activating it through dephosphorylation. This activation promotes the conversion of pyruvate to acetyl-CoA, playing a vital role in the tricarboxylic acid cycle (TCA cycle) and energy metabolism. In mouse embryonic stem cells (mESCs) differentiating into cardiomyocytes, PDP1 expression significantly decreases, leading to reduced mitochondrial function and energy metabolism.

Target/Biomarker Mouse PDP
Target Synonym PDP1; PDH; PPM2C; Protein Phosphatase 2C Magnesium-Dependent Catalytic Subunit; Pyruvate Dehyrogenase Phosphatase Catalytic Subunit 1
Gene ID 381511
UniProt ID Q3UV70

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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