Mouse Pulmonary Artery Smooth Muscle Cells-Immortalized by SV40T Antigens

The pulmonary artery originates from the right ventricle and runs obliquely upwards and backwards in front of the aorta. Below the aortic arch, it divides into the left and right pulmonary arteries, which enter the lungs via the hilum. Although the pulmonary artery is an artery, it carries deoxygenated (venous) blood because it connects to the right ventricle, which pumps deoxygenated blood. The calcium channels expressed by these cells have ICAM-1 and VCAM-1 expressed on their surface, which participate in vascular wall inflammatory responses. In vitro cultured pulmonary artery smooth muscle cells appear spindle-shaped, star-shaped, or irregular. Mouse Pulmonary Artery Smooth Muscle Cells-Immortalized by SV40T Antigens were immortalized through lentiviral transfection carrying the SV40 gene (these cells acquire puromycin resistance after transfection).