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Mouse Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit-Sandwich

Cat. No.EK5F276

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse PAPPA in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Pregnancy Associated Plasma Protein A (PAPPA).
Target Species Mouse
Species Reactivity Mouse
Detection Range 78.25-5000 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Pappalysin-1 is a metalloproteinase that specifically cleaves IGFBP-4 and IGFBP-5, releasing bound IGF. The presence of IGF greatly enhances the cleavage of IGFBP-4, while it slightly inhibits the cleavage of IGFBP-5. Isoform 2 cleaves IGFBP-4 much more slowly than PAPP-A, but its ability to cleave IGFBP-5 remains unaffected. Mice with a knockout of the PAPPA gene exhibit a significantly extended lifespan and healthspan, living on average 30%-40% longer than wild-type mice, without any abnormalities in dietary intake or endocrine function. Additionally, PAPPA-deficient mice experience lower tumor incidence and fewer age-related lesions as they age.

Target/Biomarker Mouse PAPPA
Target Synonym PAPP-A; PAPA; ASBABP2; DIPLA1; IGFBP-4ase; PAPPA1; Pappalysin 1; Insulin-Like Growth Factor Binding Protein-4 Protease; Differentially Placenta 1 Expressed Protein
Gene ID 18491
UniProt ID Q8R4K8

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Vitale, Giovanni et al. "ROLE of IGF-1 System in the Modulation of Longevity: Controversies and New Insights From a Centenarians' Perspective." Frontiers in endocrinology vol. 10 27. 1 Feb. 2019.
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