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Mouse Lung Epithelial Cell Line-MLE-12

Product Overview

The Mouse Lung Epithelial Cell Line-MLE-12 was established in 1992 from SV40 large T-antigen transgenic mice under the control of the human surfactant protein C gene promoter region. The cells produce tumors in nude mice within 6 to 9 months and express lung surfactant proteins B and C (SP-B, SP-C), along with mRNA for the large T-antigen. Lung surfactant proteins B and C were detected. The cells secrete phospholipids in response to phorbol esters and ATP, but not to forskolin.

Specifications

Cell Product Type Cell Line

Species Mouse

Cell Type Epithelial Cells

Source Lung

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium DMEM/F12; 1% L-Glutamine; 1% HEPES; 1% ITS; 10nM Hydrocortisone; 10nM Estradiol; 2% FBS; 1% Penicillin-Streptomycin Solution

Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.