Mouse Lipase, Monoacylglycerol (MGL) ELISA Kit-Sandwich

Monoglyceride lipase catalyzes the conversion of monoacylglycerides into free fatty acids and glycerol. It plays a key role in hydrolyzing the endocannabinoid 2-arachidonoylglycerol (2-AG), thereby regulating endocannabinoid signaling and influencing nociception and pain perception. In mice deficient in MGL (MGL-ko), there is an accumulation of triglycerides in adipose tissue and a significant reduction in glycerol and fatty acid levels, indicating a severe impairment in lipolysis. This lipolysis defect is partially compensated by hormone-sensitive lipase (HSL), but full restoration of lipolysis function is not achieved. MGL-ko mice exhibit markedly elevated 2-AG levels in the brain, liver, and adipose tissue, while showing decreased sensitivity of CB1 receptors, possibly due to receptor desensitization from excessive 2-AG accumulation. In the liver of MGL-ko mice, triglyceride levels decrease, along with reduced release of glycerol and fatty acids and diminished secretion of very low-density lipoprotein (VLDL), suggesting that the absence of MGL affects lipid metabolism in the liver.