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Mouse Lactate Dehydrogenase (LDH) ELISA Kit-Sandwich

Cat. No.EK5F394

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Lactate Dehydrogenase (LDH) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse LDH in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Lactate Dehydrogenase (LDH).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Lactate dehydrogenase (LDH) is a crucial oxidoreductase enzyme primarily involved in the reversible conversion of pyruvate to lactate during anaerobic glycolysis. In mice, LDH activity is regulated by various factors, including hypoxia, which induces the expression of LDH-A and subsequently promotes lactate production. This metabolic shift, known as the "Warburg effect," is notably prominent in tumor cells, which often rely on increased glycolysis for energy production, even when sufficient oxygen is available. As mice age, the A/B isoform ratio of LDH in the brain shifts, leading to elevated lactate levels. This alteration is linked to mitochondrial dysfunction and neuronal damage, potentially accelerating cognitive decline.

Target/Biomarker Mouse LDH
Target Synonym Lactate Dehydrogenase

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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