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Mouse Interleukin 12A (IL12A) ELISA Kit-Sandwich

Cat. No.EK5F399

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Interleukin 12A (IL12A) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse IL12A in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Interleukin 12A (IL12A).
Target Species Mouse
Species Reactivity Mouse
Detection Range 15.7-1000 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Interleukin-12 subunit alpha (IL12A) combines with IL12B to form the IL-12 cytokine, or with EBI3/IL27B to create the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells, dendritic cells (DCs), macrophages, and granulocytes. It plays a crucial role in regulating T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), supports the differentiation of T-helper 1 (Th1) cells, and serves as an essential link between innate resistance and adaptive immunity. IL12A is important in sepsis-induced cardiac dysfunction and may influence cardiac function by regulating the balance of M1/M2 macrophages.

Target/Biomarker Mouse IL12A
Target Synonym IL-12A;p35; IL12-A; CLMF1; NFSK; NKSF1; Natural Killer Cell Stimulatory Factor 1; Cytotoxic Lymphocyte Maturation Factor 1; NF Cell Stimulatory Factor Chain 1
Gene ID 16159
UniProt ID P43431

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Wang, Zhen et al. "Il12a Deletion Aggravates Sepsis-Induced Cardiac Dysfunction by Regulating Macrophage Polarization." Frontiers in pharmacology vol. 12 632912. 2 Jul. 2021.
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