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Mouse Hexokinase 2 (HK2) ELISA Kit-Sandwich

Cat. No.EK6F593

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Hexokinase 2 (HK2) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse HK2 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Hexokinase 2 (HK2).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Hexokinase-2 facilitates the phosphorylation of hexoses like D-glucose and D-fructose into hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). It initiates the glycolytic pathway by phosphorylating D-glucose to produce D-glucose 6-phosphate. In mice, the absence of HK2 results in disrupted glucose metabolism, characterized by impaired insulin signaling and decreased glucose utilization, mirroring features of type 2 diabetes. Additionally, diminished HK2 expression in adipose tissue contributes to hyperglycemia and insulin resistance, underscoring its vital role in maintaining glucose homeostasis. In models of inflammation and brain injury, HK2 expression is significantly upregulated in microglia.

Target/Biomarker Mouse HK2
Target Synonym HK II; Muscle form hexokinase
Gene ID 15277
UniProt ID O08528

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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