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Mouse Heat Shock Transcription Factor 1 (HSF1) ELISA Kit-Sandwich

Cat. No.EK5F8

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Heat Shock Transcription Factor 1 (HSF1) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse HSF1 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Heat Shock Transcription Factor 1 (HSF1).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.32-20 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Heat shock factor protein 1 acts as a stress-responsive, DNA-binding transcription factor that is crucial for initiating the heat shock response (HSR). This response results in the production of numerous molecular chaperones, known as heat shock proteins (HSPs), which help shield cells from damage due to stress. In cells not under stress, heat shock factor protein 1 exists in an inactive, non-DNA-binding monomeric form, within a multichaperone complex that includes HSP90. Studies have indicated that elevated temperatures sustain the decline phase of heat shock factor 1 (HSF1), which in turn activates the expression of heat shock proteins. This process has been linked to reducing insulin resistance in the skeletal muscle tissue of diet-induced obese mice.

Target/Biomarker Mouse HSF1
Target Synonym HSTF1; Heat shock factor protein 1
Gene ID 15499
UniProt ID P38532

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Wang, Qiankun et al. "High temperature ameliorates high-fat diet-induced obesity by promoting ceramide breakdown in skeletal muscle tissue." Life metabolism vol. 3,5 loae012. 3 Apr. 2024.
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