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Mouse Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit-Sandwich

Cat. No.EK5F761

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse GCLM in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Glutamate Cysteine Ligase, Modifier Subunit (GCLM).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

The mouse glutamate-cysteine ligase regulatory subunit (GCLM) is crucial for glutathione synthesis, antioxidant defense, and the onset and progression of various diseases. The GCLM gene is located in the chromosome 1p21-p22 region. GCLM serves as the regulatory subunit of glutamate-cysteine ligase (γ-glutamylcysteine synthetase), which is a key enzyme in the glutathione biosynthesis pathway. Together with the catalytic subunit (GCLC), it forms the GCL complex. GCLM influences glutathione synthesis by modulating the catalytic properties of GCLC. Its expression is regulated by multiple signaling pathways, including the activation of antioxidant response elements (AREs) like Nrf2 (nuclear factor Erythroid 2-related factor 2) and NF-κB.

Target/Biomarker Mouse GCLM
Target Synonym GLCLR; GECS; GCL; Gamma Glutamylcysteine Synthetase; Glutamate Cysteine Ligase Regulatory; Gamma-ECS regulatory subunit; Glutamate cysteine ligase modifier subunit
Gene ID 14630
UniProt ID O09172

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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