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Mouse General Transcription Factor IIF, Polypeptide 1 (GTF2F1) ELISA Kit-Sandwich

Cat. No.EK5F841

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse General Transcription Factor IIF, Polypeptide 1 (GTF2F1) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse GTF2F1 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse General Transcription Factor IIF, Polypeptide 1 (GTF2F1).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

TFIIF is a general transcription initiation factor that associates with RNA polymerase II to help its recruitment to the initiation complex, working in conjunction with TFIIB. It also facilitates transcription elongation. In mice, GTF2F1 (General Transcription Factor IIF subunit 1) is primarily involved in transcriptional regulation. It serves as a general transcription factor in primordial germ cells, playing a critical role in their formation. Research indicates that GTF2F1 enhances primordial germ cell formation by modulating the Jak/Stat3 signaling pathway and collaborates with molecules like ANXA7 and SDC1 to participate in the regulatory mechanisms of primordial germ cell formation in mouse ovaries.

Target/Biomarker Mouse GTF2F1
Target Synonym GTF2-F1; BTF4; RAP74; TF2F1; TFIIF; transcription factor IIF 74 kDa subunit
Gene ID 98053
UniProt ID Q3THK3

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Tan, Hang-Jing et al. "Single-cell RNA-seq identified novel genes involved in primordial follicle formation." Frontiers in endocrinology vol. 14 1285667. 11 Dec. 2023.
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