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Mouse Gamma-enolase (NSE) ELISA Kit-Sandwich

Cat. No.EK5F481

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Gamma-enolase (NSE) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse NSE in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Gamma-enolase (NSE).
Target Species Mouse
Species Reactivity Mouse
Detection Range 78.25-5000 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Gamma-enolase exhibits neurotrophic and neuroprotective properties across a wide range of central nervous system (CNS) neurons. It binds to cultured neocortical neurons in a calcium-dependent manner, promoting their survival. Eno2 is expressed in neurons and is linked to various neurodegenerative diseases. For instance, in the Ugt1 knockout mouse model, Eno2 overexpression is associated with neuronal cell death in the brain, potentially related to bilirubin-induced oxidative stress. Additionally, in a mouse model of aneurysm formation, Eno2 influences cell fate by regulating autophagy-related pathways, such as PI3K and MAPK/ERK26.

Target/Biomarker Mouse NSE
Target Synonym ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
Gene ID 13807
UniProt ID P17183

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Bortolussi, G et al. "Impairment of enzymatic antioxidant defenses is associated with bilirubin-induced neuronal cell death in the cerebellum of Ugt1 KO mice." Cell death & disease vol. 6,5 e1739. 7 May. 2015.
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