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Mouse Eosinophil Chemotactic Factor (ECF) ELISA Kit-Sandwich

Cat. No.EK5F161

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Eosinophil Chemotactic Factor (ECF) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse ECF in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Eosinophil Chemotactic Factor (ECF).
Target Species Mouse
Species Reactivity Mouse
Detection Range 7.82-500 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Initially, ECF was thought to be a chemokine linked to human basophils and mast cells, but later research revealed that it can also be released from mouse eosinophils and exhibits high specificity for eosinophils. Mouse eosinophils stimulated by the calcium ionophore A23187 can release ECF in a time- and dose-dependent manner. Moreover, ECF is expressed at elevated levels in mouse eosinophils, particularly in tissues such as the lungs, spleen, and bone marrow. In mouse models, ECF is associated with a variety of pathological conditions. For instance, in a mouse model of allergic asthma, ECF-L (a form of ECF in mice) is produced by T lymphocytes and plays a crucial role in the development of airway hyperresponsiveness (AHR).

Target/Biomarker Mouse ECF
Target Synonym CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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