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Mouse Endothelin 1 (ET-1) ELISA Kit-Sandwich

Cat. No.EK5F427

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Endothelin 1 (ET-1) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse ET-1 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Endothelin 1 (ET-1).
Target Species Mouse
Species Reactivity Mouse
Detection Range 7.82-500 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Endothelins are peptides derived from the endothelium that act as vasoconstrictors. Endothelin-1 likely serves as a ligand for the G-protein coupled receptors EDNRA and EDNRB, triggering the activation of PTK2B, BCAR1, BCAR3, and the GTPases RAP1 and RHOA cascade in glomerular mesangial cells. In mice, ET-1 engages in various biological roles, including vasoconstriction, inflammatory response, tissue repair, and neural regulation. Specifically, ET-1 induces neurogenic itch in mice by binding to the ETA (endothelin A receptor). Additionally, ET-1 influences sodium excretion via ETA receptors in the renal medulla, impacting blood pressure and fluid balance.

Target/Biomarker Mouse ET-1
Target Synonym ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Gene ID 13614
UniProt ID P22387

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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