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Mouse Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) ELISA Kit-Sandwich

Cat. No.EK6F587

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse DDOST in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit is part of the oligosaccharyl transferase (OST) complex, which facilitates the initial transfer of a specific glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. This process represents the first step in protein N-glycosylation. In mice, DDOST is expressed at low levels, particularly in the brain. A loss or dysfunction of DDOST can result in various genetic diseases, such as congenital disorders of glycosylation.

Target/Biomarker Mouse DDOST
Target Synonym AGE-R1; OK/SW-cl.45; OST; OST48; WBP1; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; Oligosaccharyl transferase 48 kDa
Gene ID 13200
UniProt ID O54734

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Tu, Yi et al. "MITA oligomerization upon viral infection is dependent on its N-glycosylation mediated by DDOST." PLoS pathogens vol. 18,11 e1010989. 30 Nov. 2022.
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