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Mouse Dihydroorotate Dehydrogenase (DHODH) ELISA Kit-Sandwich

Cat. No.EK6F340

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Dihydroorotate Dehydrogenase (DHODH) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse DHODH in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Dihydroorotate Dehydrogenase (DHODH).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Dihydroorotate dehydrogenase (quinone), located in the mitochondria, catalyzes the conversion of dihydroorotate to orotate, using quinone as an electron acceptor. This enzyme is essential for UMP biosynthesis via the de novo pathway. In mice, DHODH plays roles in metabolic regulation, cancer therapy, immunomodulation, antiparasitic activity, and influences various physiological and pathological processes. DHODH inhibitors have been used in mice to explore their metabolic effects. For instance, in db/db mice, DHODH inhibitors enhanced glycemic control by reducing food intake and polydipsia, improving fasting blood glucose levels, and lowering non-fasting blood glucose and HbA1c levels.

Target/Biomarker Mouse DHODH
Target Synonym DHOdehase; Dihydroorotate oxidase; Dihydroorotate dehydrogenase (quinone); mitochondrial
Gene ID 56749
UniProt ID O35435

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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