Mouse Colon Mucosal Epithelial Cells-Immortalized by SV40T Antigens

The colon extends from the cecum in the right iliac fossa and connects to the rectum at the level of the third sacral vertebra. The colon is divided into four parts: the ascending colon, transverse colon, descending colon, and sigmoid colon. Most of it is fixed to the posterior abdominal wall, and the arrangement of the colon closely resembles the letter "M," enclosing the small intestine. A transverse section of the colon, from innermost to outermost, consists of: the mucosa (epithelial layer, lamina propria, muscularis mucosae), submucosa, muscularis propria, and adventitia. In vitro culture of colonic mucosal epithelial cells provides the premise and foundation for further research into diseases such as colorectal cancer. Mouse Colon Mucosal Epithelial Cells-Immortalized by SV40T Antigens were generated via lentiviral transfection carrying the SV40 gene (these cells acquire puromycin resistance after transfection).