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Mouse Colon Mucosal Epithelial Cells-Immortalized by SV40T Antigens

Cat. No.CC7F227

Product TypeAnimal Cells and Cell Lines

Product Overview

The colon extends from the cecum in the right iliac fossa and connects to the rectum at the level of the third sacral vertebra. The colon is divided into four parts: the ascending colon, transverse colon, descending colon, and sigmoid colon. Most of it is fixed to the posterior abdominal wall, and the arrangement of the colon closely resembles the letter "M," enclosing the small intestine. A transverse section of the colon, from innermost to outermost, consists of: the mucosa (epithelial layer, lamina propria, muscularis mucosae), submucosa, muscularis propria, and adventitia. In vitro culture of colonic mucosal epithelial cells provides the premise and foundation for further research into diseases such as colorectal cancer. Mouse Colon Mucosal Epithelial Cells-Immortalized by SV40T Antigens were generated via lentiviral transfection carrying the SV40 gene (these cells acquire puromycin resistance after transfection).

Specifications

Cell Product Type Cell Line
Species Mouse
Cell Type Immortalized Cell Line
Source Colon
Growth Information Adherent; 37°C; 5%CO2
Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)
Physical State Frozen cells

Target Information

These cells are transformed and stably express the large T-antigen of the simian virus 40 (SV40T antigens) to immortalize the cells and extend their lifespan.

Target/Biomarker SV40T antigens
Target Synonym large T antigen [ Simian virus 40 ]
Gene ID 1489531

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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