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Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit-Sandwich

Cat. No.EK5F630

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse BRCA2 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Breast Cancer Susceptibility Protein 2 (BRCA2).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

The Breast cancer type 2 susceptibility protein homolog (BRCA2) is essential for repairing double-strand breaks and facilitating homologous recombination. It interacts with RAD51 and enhances recombinational DNA repair by promoting RAD51 assembly on single-stranded DNA (ssDNA). BRCA2 targets RAD51 to ssDNA rather than double-stranded DNA, enabling RAD51 to replace replication protein-A (RPA) on ssDNA and stabilizing RAD51-ssDNA filaments by preventing ATP hydrolysis. In mice, BRCA2 plays a crucial role in reproduction. Research indicates that a deficiency in BRCA2 can lead to spermatogenesis disorders, specifically preventing sperm cells from proceeding to the second phase of meiosis (the first polar phase), ultimately resulting in infertility.

Target/Biomarker Mouse BRCA2
Target Synonym BRCA2; BRCC2; FACD; FAD; FAD1; FANCD; FANCD1; Breast Cancer 2;Early Onset; Breast Cancer Type 2 Susceptibility Protein; Fanconi anemia group D1 protein
Gene ID 12190
UniProt ID P97929

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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