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Mouse Autophagy Related Protein 7 (ATG7) ELISA Kit-Sandwich

Cat. No.EK6F692

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Autophagy Related Protein 7 (ATG7) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse ATG7 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Autophagy Related Protein 7 (ATG7).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Ubiquitin-like modifier-activating enzyme ATG7 is an E1-like activating enzyme involved in two ubiquitin-like systems essential for the cytoplasm to vacuole transport (Cvt) pathway and autophagy. It activates ATG12 for its conjugation with ATG5 and the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Mice lacking Atg7 exhibit skeletal dysplasia, reduced bone mineral density, and impaired differentiation of bone marrow cells. These issues are closely tied to defective autophagy in osteoblasts, resulting in increased mitochondrial stress and apoptosis. Additionally, Atg7 deficiency impacts mitochondrial function in skeletal muscle, leading to heightened mitochondrial swelling and increased levels of protein carbonylation.

Target/Biomarker Mouse ATG7
Target Synonym APG7L; GSA7; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Ubiquitin-activating enzyme E1-like protein
Gene ID 74244
UniProt ID Q9D906

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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