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Mouse Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit-Sandwich

Cat. No.EK6F276

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse ARNT in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

The Aryl hydrocarbon receptor nuclear translocator (ARNT) is essential for the activity of the Ah (dioxin) receptor. It facilitates the movement of the ligand-binding subunit from the cytosol to the nucleus following ligand binding. This complex then triggers the transcription of genes involved in activating PAH procarcinogens. ARNT is pivotal for maintaining glucose and lipid balance in the mouse liver. Research indicates that mice with a hepatocyte-specific knockout of ARNT (LARNT) show symptoms such as hyperglycemia, decreased insulin sensitivity, lower hepatic ATP levels, and elevated postprandial serum triglyceride levels. These alterations closely resemble the symptoms observed in individuals with type 2 diabetes and metabolic syndrome.

Target/Biomarker Mouse ARNT
Target Synonym HIF-1beta; bHLHe2; Hypoxia Inducible Factor 1 Beta; Class E basic helix-loop-helix protein 2; Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta
Gene ID 11863
UniProt ID P53762

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Scott, Christopher H et al. "Hepatic Aryl hydrocarbon Receptor Nuclear Translocator (ARNT) regulates metabolism in mice." PloS one vol. 12,11 e0186543. 30 Nov. 2017.
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