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Mouse Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit-Sandwich

Cat. No.EK5F617

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse ALOX5 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Arachidonate-5-Lipoxygenase (ALOX5).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Polyunsaturated fatty acid 5-lipoxygenase catalyzes the oxygenation of arachidonate to 5-hydroperoxyeicosatetraenoate (5-HPETE), followed by its dehydration to 5,6-epoxyeicosatetraenoate (Leukotriene A4/LTA4). These steps are the initial stages in the biosynthesis of leukotrienes, which are powerful inflammation mediators. In mice, the Alox5 gene is pivotal in the pathogenesis of chronic myeloid leukemia (CML). Research indicates that loss or inhibition of Alox5 significantly impacts the function of leukemia stem cells (LSCs), leading to their inability to form tumors in the BCR-ABL-induced mouse CML model. Specifically, Alox5-deficient mouse LSCs lose their normal self-renewal capability and cannot effectively invade the bone marrow of recipient mice.

Target/Biomarker Mouse ALOX5
Target Synonym LOX5; 5-LOX; ALOX-5; 5-LO; 5LPG; LOG5; Lipoxygenase 5
Gene ID 11689
UniProt ID P48999

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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