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Mouse Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit-Sandwich

Cat. No.EK5F493

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Mouse Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit-Sandwich is designed for the quantitative determination of Mouse Alk-Smase in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Mouse Alkaline Sphingomyelinase (Alk-Smase).
Target Species Mouse
Species Reactivity Mouse
Detection Range 0.17-10 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Alkaline sphingomyelinase (al-SMase) is a crucial enzyme found in the intestine, primarily responsible for generating ceramide by hydrolyzing sphingomyelin. This process holds significant physiological importance across various organisms, as it regulates cell proliferation, apoptosis, and inflammatory responses. Research indicates that when the activity of alkaline sphingomyelinase is diminished in the intestines of mice, there is a marked increase in the accumulation of undigested sphingomyelin, accompanied by a decrease in ceramide production, ultimately leading to compromised intestinal barrier function. Besides its essential role in maintaining intestinal health, alkaline sphingomyelinase also contributes to the regulation of inflammatory responses.

Target/Biomarker Mouse Alk-Smase
Target Synonym ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase
UniProt ID Q3TIW9

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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