Mouse Acetyl Coenzyme A Carboxylase Alpha (ACACa) ELISA Kit-Sandwich

Acetyl-CoA carboxylase 1 (ACC1) is a cytosolic enzyme responsible for catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, which is the initial and rate-limiting step in de novo fatty acid biosynthesis. This process involves a two-step reaction that begins with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. In mouse hepatocytes, ACC1 is primarily found in the mitochondrial matrix, but it may localize to the cytoplasm or endoplasmic reticulum in other tissues. ACC1 plays a critical role in regulating the biosynthesis and metabolism of fatty acids by catalyzing the production of malonyl-CoA from acetyl-CoA in mice, contributing to various physiological and pathological processes. In mouse models, the knockout of the ACC1 gene results in embryonic lethality, underscoring its vital role in embryonic development.