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Fish Proline Hydroxylase (PHD) ELISA Kit-Sandwich

Cat. No.EK11F386

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Fish Proline Hydroxylase (PHD) ELISA Kit-Sandwich is designed for the quantitative determination of Fish Proline Hydroxylase (PHD) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Fish PHD.
Target Species Aquaculture Species
Species Reactivity Aquaculture Species
Recovery 98%-102%
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%
Assay Time Around 180 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids; 10 μL

Target Information

Prolyl Hydroxylase, also known as procollagen-proline dioxygenase, belongs to the group of enzymes called alpha-ketoglutarate-dependent hydroxylases. These enzymes facilitate the incorporation of oxygen into organic compounds through a process that involves alpha-Ketoglutaric acid, Fe2+, and ascorbate. The oxygen-dependent hydroxylation of hypoxia-inducible factor (HIF)-α subunits by prolyl hydroxylase domain (PHD) proteins serves as a signal for their polyubiquitination and subsequent proteasomal degradation. This process plays a crucial role in regulating HIF abundance and maintaining oxygen homeostasis.

Target/Biomarker Fish PHD
Target Synonym Proline hydroxylase; ProHydroxylase; PHD

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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