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Fish Aminopyrine N Demethylase (APND) ELISA Kit-Sandwich

Cat. No.EK11F353

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Fish Aminopyrine N Demethylase (APND) ELISA Kit-Sandwich is designed for the quantitative determination of Fish Aminopyrine N Demethylase (APND) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Fish APND.
Target Species Aquaculture Species
Species Reactivity Aquaculture Species
Detection Range 16-550 mIU/mL
Sensitivity 4 mIU/mL
Recovery 98%-102%
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%
Assay Time Around 180 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids; 10 μL

Target Information

Aminopyrine N Demethylase (APND) is a crucial enzyme within the cytochrome P450 family. It plays a significant role in metabolizing exogenous substances, particularly drugs and poisons. Functionally equivalent to isoform CYP3A4, APND is closely associated with the methylation reactions of drugs. Its unique properties include a molecular weight of approximately 49 kD, a CO-peak maximum at 450.5 nm, and a high demethylation rate for aminopyrine and benzphetamine. This enzyme's impact on drug metabolism underscores its importance in pharmacokinetics and drug interactions.

Target/Biomarker Fish APND
Target Synonym Aminopyrine N Demethylase; APND

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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