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Feline 4-Hydroxynonenal (4-HNE) ELISA Kit-Competitive

Product Overview

BioVenic Feline 4-Hydroxynonenal (4-HNE) ELISA Kit-Competitive is designed for the quantitative determination of Feline 4-Hydroxynonenal (4-HNE) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Competitive ELISA method. For research use only.

Specifications

Assay Type ELISA-Competitive

Specificity The assay kit is specific for Feline 4-HNE.

Target Species Feline

Species Reactivity Feline

Detection Range 0.64-40 ng/mL

Sensitivity 0.39 ng/mL

Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%

Assay Time Around 120 min

Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

4-Hydroxynonenal (4-HNE), also known as 4-hydroxy-2E-nonenal, is an α,β-unsaturated hydroxyalkenal produced by lipid peroxidation in cells. 4-HNE is the major α,β-unsaturated hydroxyalkenal formed during lipid peroxidation. It is generated during the oxidation of lipids containing polyunsaturated omega-6 fatty acids, such as arachidonic and linoleic acids. 4-HNE is hypothesized to play a key role in cell signal transduction across various pathways, from cell cycle events to cellular adhesion. It contributes to oxidative stress-mediated deterioration of the ageing oocyte.

Target/Biomarker Feline 4-HNE

Target Synonym 4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

Mihalas, B. P. et al. The lipid peroxidation product 4-hydroxynonenal contributes to oxidative stress-mediated deterioration of the ageing oocyte. Scientific reports. 2017, 7: 6247.