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Chinese Hamster Ovary Cells-CHO-dhfr

Product Overview

Chinese Hamster Ovary Cells-CHO-dhfr (dihydrofolate reductase deficient) are cells that lack dihydrofolate reductase. Without HT (hypoxanthine-thymidine), these cells will die. Adding methotrexate to the cell culture medium can prevent the growth of revertant cells with low drug resistance.

Specifications

Cell Product Type Cell Line

Species Hamster

Cell Type Epithelial Cells

Source Ovarian

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium IMDM with 10% FBS; 1% Penicillin-Streptomycin Solution; 0.05 mM Hypoxanthine; 0.016 mM Thymine; 100 nM Methotrexate

Physical State Frozen cells

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.