Canine Myocardial Fibroblasts-Immortalized by SV40T Antigens

In dog myocardial tissue, cardiac fibroblasts (CFs) are one of the primary interstitial cell types. In a normal heart, cardiac fibroblasts predominantly exist in a quiescent state, expressing vimentin. However, under pathological conditions (such as myocardial infarction, heart failure, cardiomyopathy, etc.), they become activated and express α-SMA, transforming into myofibroblasts. Cardiac fibroblasts constitute approximately 60%-70% of the total cells in normal myocardial tissue, making them the main component of non-myocardial cells in the heart. They are widely distributed throughout the cardiac tissue, encapsulating cardiomyocytes and connecting the myocardial interstitium. They are closely associated with pathological conditions like ischemic heart disease, inflammation, hypertrophy, and infarction. Canine Myocardial Fibroblasts-Immortalized by SV40T Antigens were immortalized through lentiviral transfection carrying the SV40 gene (these cells acquire puromycin resistance after transfection).