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Canine 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit-Competitive

Product Overview

BioVenic Canine 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit-Competitive is designed for the quantitative determination of Canine 12-Hydroxyeicosatetraenoic Acid (12-HETE) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Competitive ELISA method. For research use only.

Specifications

Assay Type ELISA-Competitive

Specificity The assay kit is specific for Canine 12-HETE.

Target Species Canine

Species Reactivity Canine

Detection Range 15.63-1000 ng/mL

Sensitivity 5.32 ng/mL

Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%

Assay Time Around 120 min

Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

12-Hydroxyeicosatetraenoic acid (12-HETE) is a derivative of the 20 carbon polyunsaturated fatty acid, arachidonic acid. 12-HETE acts locally as an autocrine or paracrine signaling agent. It can regulate cellular responses and tissue behavior. Rabbit, chicken, rat, and dog erythrocytes (10(9) cells/ml) synthesized immunologically active 12-HETE when stimulated by the Ca²⁺ ionophore, A-23187. Notably, 12-HETE is also associated with inflammation.

Target/Biomarker Canine 12-HETE

Target Synonym 12HETE

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.