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Bovine D-3-Phosphoglycerate Dehydrogenase (PHGDH) ELISA Kit-Sandwich

Cat. No.EK12F26

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Bovine D-3-Phosphoglycerate Dehydrogenase (PHGDH) ELISA Kit-Sandwich is designed for the quantitative determination of Bovine D-3-Phosphoglycerate Dehydrogenase (PHGDH) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Bovine PHGDH.
Target Species Bovine
Species Reactivity Bovine
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%
Assay Time Around 270 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

Bovine D-3-Phosphoglycerate Dehydrogenase is encoded by the PHGDH gene in cattle. This enzyme is essential for the phosphorylated pathway of L-serine biosynthesis. It catalyzes the committed step in de novo serine biosynthesis. Inhibition of PHGDH induces alterations in nucleotide metabolism independent of serine utilization. Dysregulation of PHGDH can impact L-serine availability and contribute to various diseases, including cancer and neurological disorders.

Target/Biomarker Bovine PHGDH
Target Synonym phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; 2-oxoglutarate reductase; 3-PGDH; malate dehydrogenase
Gene ID 505103
UniProt ID Q5EAD2

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Reid, M. A. et al. Serine synthesis through PHGDH coordinates nucleotide levels by maintaining central carbon metabolism. Nature communications. 2018, 9: 5442.
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