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Bovine Protein Disulfide-Isomerase (P4HB) ELISA Kit-Sandwich

Cat. No.EK11F678

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Bovine Protein Disulfide-Isomerase (P4HB) ELISA Kit-Sandwich is designed for the quantitative determination of Bovine Protein Disulfide-Isomerase (P4HB) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Bovine P4HB.
Target Species Bovine
Species Reactivity Bovine
Detection Range 0.312-20 ng/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%
Assay Time Around 270 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

Protein disulfide isomerase (PDI), is a member of the thioredoxin superfamily of redox proteins. PDI has three catalytic activities including, thiol-disulfide oxireductase, disulfide isomerase and redox-dependent chaperone. Originally, PDI was identified in the lumen of the endoplasmic reticulum and subsequently detected at additional locations, such as cell surfaces and the cytosol. Bovine Protein Disulfide-Isomerase is encoded by the P4HB gene in cattle. It is a protein involved in protein folding, and it is anti-apoptotic.

Target/Biomarker Bovine P4HB
Target Synonym prolyl 4-hydroxylase subunit beta; PDI; protein disulfide-isomerase; PDI (E.C.5.3.4.1); cellular thyroid hormone binding protein; p55; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase beta polypeptide; prolyl 4-hydroxylase, beta subunit
Gene ID 281373
UniProt ID P05307

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. You, J. et al. Treatment with the Proteasome Inhibitor MG132 during the End of Oocyte Maturation Improves Oocyte Competence for Development after Fertilization in Cattle. PloS one. 2012, 7: e48613.
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