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Bovine Cyclin-Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit-Sandwich

Cat. No.EK12F341

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Bovine Cyclin-Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit-Sandwich is designed for the quantitative determination of Bovine Cyclin-Dependent Kinase Inhibitor 1A (CDKN1A) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Bovine CDKN1A.
Target Species Bovine
Species Reactivity Bovine
Detection Range 78.125-5000 pg/mL
Sensitivity 46.875 pg/mL
Recovery 86%-104%
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%
Assay Time Around 270 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

Bovine cyclin-dependent kinase inhibitor 1A is encoded by the CDKN1A gene in cattle. This gene encodes a potent inhibitor of cyclin-dependent kinase. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or cyclin-dependent kinase4 complexes and thus acts as a regulator of cell cycle progression in G1. The expression of this gene is tightly controlled by the tumour suppressor protein p53, which mediates p53-dependent G1 cell cycle arrest in response to a variety of stress stimuli. It was found to be remarkably differentially expressed between the mammary glands of lactating Holstein cows with extremely high and low milk protein and fat content.

Target/Biomarker Bovine CDKN1A
Target Synonym cyclin dependent kinase inhibitor 1A; p21; CIP1; SDI1; WAF1; cyclin-dependent kinase inhibitor 1
Gene ID 513497
UniProt ID A5PKM2

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Han, B. et al. Determination of genetic effects of ATF3 and CDKN1A genes on milk yield and compositions in Chinese Holstein population. BMC genetics. 2017, 18: 47.
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